Peptidylglycine amidating monooxygenase russian best dating


Peptidylglycine-α-amidating monooxygenase (PAM) may play a role in the secretion of atrial natriuretic peptide (ANP), which is a hormone involved in the maintenance of blood pressure (BP).The objective of the present study was to determine whether PAM is a novel candidate gene for hypertension (HTN).Serum fasting high-density lipoprotein (HDL)-cholesterol was measured using a selective inhibition method with the HDL-C Plus Kit (Roche, Mannheim, Germany).The resulting color reactions of the assays were monitored using a Hitachi 7600 autoanalyzer (Hitachi, Tokyo, Japan).


Since 2014, the Korean Chip (K-CHIP), which includes 833,535 SNPs and uses an oligomer as a probe, has been developed by the Korea Biobank Array project as a low-cost customized chip that is optimized for genetic studies of diseases and complex traits in Koreans (Additional file 1: Table S1).The gels were scanned using a GS-800 Calibrated Imaging Densitometer (Bio-Rad Laboratories, Hercules, CA, USA).Plasma ox-LDL was estimated using an enzyme immunoassay (Mercodia AB, Uppsala, Sweden), and the resulting color reaction was determined at 450 nm on a Wallac Victor2 multilabel counter (Perkin-Elmer Life Sciences, Boston, MA, USA).The neuroendocrine processing enzyme peptidylglycine-α-amidating monooxygenase (PAM) is highly concentrated in the atrium and may play a role in the secretion of atrial natriuretic peptide (ANP), which is a hormone involved in BP maintenance and fluid homeostasis [9,10,11].

Indeed, PAM and pro-ANP (the bioactive form of ANP) are the predominant membrane-associated proteins in atrial secretory granules [12].

The Friedewald formula was used to indirectly calculate low-density lipoprotein (LDL)-cholesterol levels as follows: LDL-cholesterol = TC − [HDL-cholesterol (TG/5)].


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